Monitoring of noble, signal and narrow-clawed crayfish using environmental DNA from freshwater samples
نویسندگان
چکیده
For several hundred years freshwater crayfish (Crustacea-Decapoda-Astacidea) have played an important ecological, cultural and culinary role in Scandinavia. However, many native populations of noble crayfish Astacus astacus have faced major declines during the last century, largely resulting from human assisted expansion of non-indigenous signal crayfish Pacifastacus leniusculus that carry and transmit the crayfish plague pathogen. In Denmark, also the non-indigenous narrow-clawed crayfish Astacus leptodactylus has expanded due to anthropogenic activities. Knowledge about crayfish distribution and early detection of non-indigenous and invasive species are crucial elements in successful conservation of indigenous crayfish. The use of environmental DNA (eDNA) extracted from water samples is a promising new tool for early and non-invasive detection of species in aquatic environments. In the present study, we have developed and tested quantitative PCR (qPCR) assays for species-specific detection and quantification of the three above mentioned crayfish species on the basis of mitochondrial cytochrome oxidase 1 (mtDNA-CO1), including separate assays for two clades of A. leptodactylus. The limit of detection (LOD) was experimentally established as 5 copies/PCR with two different approaches, and the limit of quantification (LOQ) were determined to 5 and 10 copies/PCR, respectively, depending on chosen approach. The assays detected crayfish in natural freshwater ecosystems with known populations of all three species, and show promising potentials for future monitoring of A. astacus, P. leniusculus and A. leptodactylus. However, the assays need further validation with data 1) comparing traditional and eDNA based estimates of abundance, and 2) representing a broader geographical range for the involved crayfish species.
منابع مشابه
Genetic variation in the narrow-clawed crayfish (Astacus leptodactylus) populations as assessed by PCR-RFLP of mitochondrial COI gene
The genetic variation and population structure of narrow-clawed crayfish (Astacus leptodactylus) was examined by means of polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) analysis of the cytochrome oxidase subunit I (COI) of mitochondrial DNA. A total of 194 adult specimens were collected from seven sample sites including, two in the south Caspian Sea and one each...
متن کاملEvaluation of different dietary protein and energy levels on growth performance and body composition of narrow clawed crayfish (Astacus leptodactylus)
متن کامل
Detection and quantification of the crayfish plague agent in natural waters: direct monitoring approach for aquatic environments.
Aphanomyces astaci, a specialised parasite of North American freshwater crayfish, is the disease agent of crayfish plague that is lethal to European freshwater crayfish. The life cycle of A. astaci has been inferred from experimental laboratory studies, but less is known about its natural sustainability and ecology. To address such questions, tools for monitoring of A. astaci directly in aquati...
متن کاملDietary Chlosterol Requirement of Juvenile Narrow-clawed Crayfish (Astacus leptodactylus) in a Recirculating Aquaculture System
Dietary cholesterol has been shown to be essential for several crustacean species. However, cholesterol requirement of narrow-clawed crayfish has not been reported. Therefore, a 12-week feeding trial was conducted to determine deitary cholesterol requirement of narrow-clawed crayfish. Juvenile crayfish with an average body weight of 13.8±1 g mean weight were intensively stocked in an indoor rec...
متن کاملFluctuation of gonadosomatic index during oocyte development in the narrow-clawed crayfish Astacus leptodactylus (Eschscholtz, 1823) in Aras Dam Lake, Iran
This study was carried out with the aim of examining the seasonal reproductive cycle of the female crayfish Astacus leptodactylus from Aras Dam Lake, Western-Azerbaijan, Iran. Gonadosomatic index (GSI), and oocyte size were measured in females sampled seasonally in June, August, November(2011), January(2012). Development of the oocytes was categorized according to the diameter and the presenc...
متن کامل